MOLECULAR CHARACTERIZATION AND MODELING OF SECONDARY STRUCTURE OF 16S rRNA FROM AEROMONAS VERONII
نویسندگان
چکیده
Aeromonas veronii is a human pathogen that causes diarrhea, wound infections and hemorrhagic septicemia. A. veronii isolated from Gomti river water and identified on basis of 16S rRNA sequences, which showed the high degree of homology with existing sequences. Moreover, the 16S rRNA sequences were also being used for modeling of RNA secondary structures and resembles with Gibbs free energy of other available strains. The result indicates the accurate molecular identification of A. veronii and its phylogeny shows the high degree of homology with diverse source of other strains.
منابع مشابه
Model-Free RNA Sequence and Structure Alignment Informed by SHAPE Probing Reveals a Conserved Alternate Secondary Structure for 16S rRNA
Discovery and characterization of functional RNA structures remains challenging due to deficiencies in de novo secondary structure modeling. Here we describe a dynamic programming approach for model-free sequence comparison that incorporates high-throughput chemical probing data. Based on SHAPE probing data alone, ribosomal RNAs (rRNAs) from three diverse organisms--the eubacteria E. coli and C...
متن کاملCharacterization of fragmented mitochondrial ribosomal RNAs of the colorless green alga Polytomella parva.
We have identified previously in mitochondrial DNA of the colorless, chlorophycean, green algal taxon, Polytomella parva, potential coding regions for four small subunit (SSU) and eight large subunit (LSU) rRNA fragments. In this study with P.parva, we isolated RNA from a mitochondrial-enriched preparation, characterized the 12 mitochondrial rRNA transcripts by either northern blot analysis or ...
متن کاملMolecular and Phylogenetic Analysis and Protein Structural modeling of NS Gene of Human Influenza A Virus Subtype H1N1 Circulating in Iran 2015 & 2017
Abstract Background: The NS (non-structural) genomic segment of influenza A virus expresses two proteins (NS1 and NS2) which are responsible for the virulence and pathogenicity of virus. In this study we investigate the characterization and variability of the NS gene recovered from H1N1 influenza viruses isolated from Iranian patients during the 2017 seasonal outbreak and from high...
متن کاملSynthesis, characterization, antibacterial activity and molecular modeling studies of Ni(II) and Zn(II) complexes with phenylpyridylformamidine ligand
The Ni(II) and Zn(II) complexes with phenylpyridylformamidine (PhPyF) ligand, [Ni(PhPyF)Cl2] and [Zn(PhPyF)Cl2], have been prepared and investigated using different chemical techniques such as elemental analysis, molar conductance, FT-IR, UV-vis spectra and magnetic moment. The obtained chemical analysis data showed the formation of 1:1 (metal: ligand) ratio. The square planar and tetrahedral g...
متن کاملMOLECULAR MODELING AND DOCKING STUDIES OF COLD ACTIVE LIPASE FROM Pseudomonas fluorescens
Molecular Modeling is essential tool in the drug design process describes the generation, manipulation or representation of 3D structures of the molecules and associated physico-chemical properties while docking predicts the preferred orientation of one molecule to a second when bound to each other to form a stable complex. A cold active lipase producing potential psychrophilic bacteria (GN) wa...
متن کامل